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Image Search Results
Journal: CNS Neuroscience & Therapeutics
Article Title: Surgical Stress Induces Brain‐Derived Neurotrophic Factor Reduction and Postoperative Cognitive Dysfunction Via Glucocorticoid Receptor Phosphorylation in Aged Mice
doi: 10.1111/cns.12368
Figure Lengend Snippet: Surgical stress elevated GR phosphorylation at ser220 by CDK5 and its regulator in prefrontal cortex of POCD mice. (A), GR phosphorylation was tested with Western blot. The phosphorylation at ser220 site was significantly elevated in POCD group (two‐tailed unpaired t‐test, P = 0.0136 for pGRs220; P = 0.2303 for pGRs212; P = 0.3860 for pGRs234), compared with control group. (B) The level of CDK5 and its regulators p35, p25 in prefrontal cortex was tested with Western blot in POCD and control mice. The level of CDK5 was upregulated in POCD group compared with control (two‐tailed unpaired t‐test, P = 0.0178). CDK5 regulators p35 (P = 0.0140), p25 (P = 0.0139) also elevated in prefrontal cortex compared with control. (C), nonparametric correlation analysis of GR phosphorylation and behavioral index in 20‐month groups (CDK5 with MWM, r2 = 0.75, P < 0.001; CDK5 with EPM, r2 = 0.78, P < 0.001; pGRs220 with MWM, r2 = 0.63, P < 0.001; pGRs220 with EPM, r2 = 0.73, P < 0.001) (C). (*P < 0.05, POCD compared with control) (n = 8 mice/group). Error bar represents SEM. (D), no significant changes on pGRs220 (P = 0.7576), CDK5 (P = 0.8640) and its regulators (P = 0.8182 for p35 and P = 0.8844 for p25) in 6‐month groups. (*P < 0.05, POCD compared with control) (n = 8 mice/group). Data are Mean ± SEM (Error bars).
Article Snippet: After transferred onto PVDF membrane, the following primary antibodies were used: sheep anti‐BDNF antibody (1:5000, millipore, Billerica, MA, USA); rabbit anti‐GR and anti‐p‐GRs220 antibody (1:12000, CST, Boston, MA, USA); rabbit anti‐CDK5 antibody (1:5000, BD, NJ, USA); rabbit
Techniques: Western Blot, Two Tailed Test